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Large depth of field microscopic imaging

MicroFocus is the software allowing to create sharp images corresponding to image from a large depth of field microscope from a sequences of microscopic images taken by a shallow depth of field microscope.

A focused image is automatically computed from a sequence of shallow depth of field images taken at different focus depths. The method makes no assumptions about the continuity of the specimen, so it can be also used to focus e.g. on a collection of particles floating in a medium.

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A part of sequence of images taken at various depths

Final large depth picture
Computed large depth of field image

MicroFocus application

Powerful application for creating large depth of field (focused) images from shallow ones. Demo version available for free!


Want to integrate the focusing algorithms into your own application? Use our MFOCUS library SDK!

To get more information about MicroFocus, check our facts sheets page. This software was developed in cooperation with CMP.